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Registro Completo |
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Biblioteca(s): |
Embrapa Agroenergia. |
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Data corrente: |
26/08/2019 |
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Data da última atualização: |
18/11/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
AZEVEDO, R.; LOPES, J. L.; SOUZA, M. M. de; QUIRINO, B. F.; JUNGMANN, L.; MARINS, L. F. |
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Afiliação: |
Raíza Azevedo, Universidade Federal do Rio Grande do Sul; Jéssika Lawall Lopes, Universidade Federal do Rio Grande do Sul; Manuel Macedo de Souza; BETANIA FERRAZ QUIRINO, CNPAE; LETICIA JUNGMANN CANCADO, CNPAE; Luis Fernando Marins, Universidade Federal do Rio Grande do Sul. |
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Título: |
Synechococcus elongatus as a model of photosynthetic bioreactor for expression of recombinant B-glucosidases. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Biotechnology for Biofuels, v.12, n. 174, 2019. |
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DOI: |
https://doi.org/10.1186/s13068-019-1505-9 |
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Idioma: |
Inglês |
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Conteúdo: |
Background: The production of glucose from cellulose requires cellulases, which are obtained from decomposing microorganisms such as fungi and bacteria. Among the cellulases, β-glucosidases convert cellobiose to glucose and have low concentration in commercial cocktails used for the production of second-generation (2G) ethanol. Genetic engineering can be used to produce recombinant β-glucosidases, and cyanobacteria may be interesting bioreactors. These photosynthetic microorganisms can be cultured using CO2 emitted from the first-generation ethanol (1G) industry as a carbon source. In addition, vinasse, an effluent of 1G ethanol production, can be used as a source of nitrogen for cyanobacteria growth. Thus, photosynthetic bioreactors cannot only produce cellulases at a lower cost, but also reduce the environmental impact caused by residues of 1G ethanol production. Results: In the present work, we produced a strain of Synechococcus elongatus capable of expressing high levels of a heterologous β-glucosidase from a microorganism from the Amazonian soil. For this, the pET system was cloned into cyanobacteria genome. This system uses a dedicated T7 RNA polymerase for the expression of the gene of interest under the control of a nickel-inducible promoter. The results showed that the pET system functions efficiently in S. elongatus, once nickel induced T7 RNA polymerase expression which, in turn, induced expression of the gene of the microbial β-glucosidase at high levels when compared with non-induced double transgenic strain. β-glucosidase activity was more than sevenfold higher in the transformed cyanobacteria than in the wild-type strain. Conclusions: The T7 system promotes high expression levels of the cloned gene in S. elongatus, demonstrating that the arrangement in which an exclusive RNA polymerase is used for transcription of heterologous genes may contribute to high-level gene expression in cyanobacteria. This work was the first to demonstrate the use of cyanobacteria for the production of recombinant β-glucosidases. This strategy could be an alternative to reduce the release of 1G ethanol by-products such as CO2 and vinasse, not only contributing to decrease the cost of β-glucosidase production, but also mitigating the environmental impacts of ethanol industrial plants. MenosBackground: The production of glucose from cellulose requires cellulases, which are obtained from decomposing microorganisms such as fungi and bacteria. Among the cellulases, β-glucosidases convert cellobiose to glucose and have low concentration in commercial cocktails used for the production of second-generation (2G) ethanol. Genetic engineering can be used to produce recombinant β-glucosidases, and cyanobacteria may be interesting bioreactors. These photosynthetic microorganisms can be cultured using CO2 emitted from the first-generation ethanol (1G) industry as a carbon source. In addition, vinasse, an effluent of 1G ethanol production, can be used as a source of nitrogen for cyanobacteria growth. Thus, photosynthetic bioreactors cannot only produce cellulases at a lower cost, but also reduce the environmental impact caused by residues of 1G ethanol production. Results: In the present work, we produced a strain of Synechococcus elongatus capable of expressing high levels of a heterologous β-glucosidase from a microorganism from the Amazonian soil. For this, the pET system was cloned into cyanobacteria genome. This system uses a dedicated T7 RNA polymerase for the expression of the gene of interest under the control of a nickel-inducible promoter. The results showed that the pET system functions efficiently in S. elongatus, once nickel induced T7 RNA polymerase expression which, in turn, induced expression of the gene of the microbial β-glucosidase at ... Mostrar Tudo |
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Palavras-Chave: |
PET system. |
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Thesaurus Nal: |
Cellulases; Cyanobacteria; Genetic engineering. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/201297/1/Synechocossus-2019.pdf
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Marc: |
LEADER 03085naa a2200241 a 4500
001 2111615
005 2019-11-18
008 2019 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1186/s13068-019-1505-9$2DOI
100 1 $aAZEVEDO, R.
245 $aSynechococcus elongatus as a model of photosynthetic bioreactor for expression of recombinant B-glucosidases.$h[electronic resource]
260 $c2019
520 $aBackground: The production of glucose from cellulose requires cellulases, which are obtained from decomposing microorganisms such as fungi and bacteria. Among the cellulases, β-glucosidases convert cellobiose to glucose and have low concentration in commercial cocktails used for the production of second-generation (2G) ethanol. Genetic engineering can be used to produce recombinant β-glucosidases, and cyanobacteria may be interesting bioreactors. These photosynthetic microorganisms can be cultured using CO2 emitted from the first-generation ethanol (1G) industry as a carbon source. In addition, vinasse, an effluent of 1G ethanol production, can be used as a source of nitrogen for cyanobacteria growth. Thus, photosynthetic bioreactors cannot only produce cellulases at a lower cost, but also reduce the environmental impact caused by residues of 1G ethanol production. Results: In the present work, we produced a strain of Synechococcus elongatus capable of expressing high levels of a heterologous β-glucosidase from a microorganism from the Amazonian soil. For this, the pET system was cloned into cyanobacteria genome. This system uses a dedicated T7 RNA polymerase for the expression of the gene of interest under the control of a nickel-inducible promoter. The results showed that the pET system functions efficiently in S. elongatus, once nickel induced T7 RNA polymerase expression which, in turn, induced expression of the gene of the microbial β-glucosidase at high levels when compared with non-induced double transgenic strain. β-glucosidase activity was more than sevenfold higher in the transformed cyanobacteria than in the wild-type strain. Conclusions: The T7 system promotes high expression levels of the cloned gene in S. elongatus, demonstrating that the arrangement in which an exclusive RNA polymerase is used for transcription of heterologous genes may contribute to high-level gene expression in cyanobacteria. This work was the first to demonstrate the use of cyanobacteria for the production of recombinant β-glucosidases. This strategy could be an alternative to reduce the release of 1G ethanol by-products such as CO2 and vinasse, not only contributing to decrease the cost of β-glucosidase production, but also mitigating the environmental impacts of ethanol industrial plants.
650 $aCellulases
650 $aCyanobacteria
650 $aGenetic engineering
653 $aPET system
700 1 $aLOPES, J. L.
700 1 $aSOUZA, M. M. de
700 1 $aQUIRINO, B. F.
700 1 $aJUNGMANN, L.
700 1 $aMARINS, L. F.
773 $tBiotechnology for Biofuels$gv.12, n. 174, 2019.
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Registro original: |
Embrapa Agroenergia (CNPAE) |
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Registro Completo
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Biblioteca(s): |
Embrapa Acre. |
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Data corrente: |
25/10/2023 |
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Data da última atualização: |
25/10/2023 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
OLIVEIRA, J. C. de; FORMIGHIERI, E. F.; GARCIA, A. L. B.; MARGARIDO, G. R. A.; CAMPOS, T. de. |
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Afiliação: |
JÔNATAS CHAGAS DE OLIVEIRA, UNIVERSIDADE FEDERAL DO ACRE; EDUARDO FERNANDES FORMIGHIERI, CNPAE; ANA LETYCIA BASSO GARCIA, ESCOLA SUPERIOR DE AGRICULTURA LUIZ DE QUEIROZ; GABRIEL RODRIGUES ALVES MARGARIDO, ESCOLA SUPERIOR DE AGRICULTURA LUIZ DE QUEIROZ; TATIANA DE CAMPOS, CPAF-AC. |
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Título: |
Caracterização funcional do transcriptoma de amendoim forrageiro. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
In: SEMINÁRIO DA EMBRAPA ACRE DE INICIAÇÃO CIENTÍFICA E PÓS-GRADUAÇÃO, 5., 2022, Rio Branco, AC. O papel da tecnologia agrícola na segurança alimentar: anais. Rio Branco, AC: Embrapa Acre, 2023. |
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Páginas: |
p. 129-133. |
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Descrição Física: |
Pôster. |
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Série: |
(Embrapa Acre. Eventos técnicos & científicos, 5). |
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Idioma: |
Português |
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Notas: |
Editores técnicos: Rodrigo Souza Santos; Fabiano Marçal Estanislau. |
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Conteúdo: |
O uso do amendoim forrageiro em consórcios com gramíneas nas pastagens e como cobertura verde, consorciado com culturas comerciais, tem crescido nos últimos anos. A análise do genoma funcional permite a identificação de genes de interesse agronômico. Assim, o objetivo deste trabalho foi realizar a anotação funcional de genes do transcriptoma de folhas de Arachis pintoi. Dos 98.432 transcritos analisados, 69% apresentaram correspondências com o banco de dados de proteínas do National Center of Biotechnology Information. As classes função molecular (36%) e processo biológico (35,8%) representaram a maioria dos termos de Gene Ontology atribuídos, enquanto o componente celular (28,2%) apresentou menor número. A análise de expressão diferencial identificou 1.550 e 1.357 genes com maior nível de expressão nas cultivares Amarillo e Belomonte, respectivamente. A análise de enriquecimento dos genes mostrou que 55,63% pertencem à classe componente celular, seguida por função molecular (26,48%) e processo biológico (20,89%). Esses resultados são o primeiro relato de anotação funcional de A. pintoi que irá fornecer uma importante fonte de informação para avanços nos estudos de expressão, silenciamento e edição gênica nos programas de melhoramento de Arachis. |
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Palavras-Chave: |
Amarillo; Amendoim forrageiro; Anotação funcional; Belomonte; Cacahuetes forrajeros; Forage peanut; Hojas; RNA-seq; Transcriptoma. |
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Thesagro: |
Folha; Genoma; Método de Análise. |
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Thesaurus NAL: |
Arachis pintoi; Genome; Leaves; Transcriptome. |
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Categoria do assunto: |
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1157482/1/27546.pdf
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Marc: |
LEADER 02585nam a2200385 a 4500
001 2157482
005 2023-10-25
008 2023 bl uuuu u00u1 u #d
100 1 $aOLIVEIRA, J. C. de
245 $aCaracterização funcional do transcriptoma de amendoim forrageiro.$h[electronic resource]
260 $aIn: SEMINÁRIO DA EMBRAPA ACRE DE INICIAÇÃO CIENTÍFICA E PÓS-GRADUAÇÃO, 5., 2022, Rio Branco, AC. O papel da tecnologia agrícola na segurança alimentar: anais. Rio Branco, AC: Embrapa Acre$c2023
300 $ap. 129-133.$cPôster.
490 $a(Embrapa Acre. Eventos técnicos & científicos, 5).
500 $aEditores técnicos: Rodrigo Souza Santos; Fabiano Marçal Estanislau.
520 $aO uso do amendoim forrageiro em consórcios com gramíneas nas pastagens e como cobertura verde, consorciado com culturas comerciais, tem crescido nos últimos anos. A análise do genoma funcional permite a identificação de genes de interesse agronômico. Assim, o objetivo deste trabalho foi realizar a anotação funcional de genes do transcriptoma de folhas de Arachis pintoi. Dos 98.432 transcritos analisados, 69% apresentaram correspondências com o banco de dados de proteínas do National Center of Biotechnology Information. As classes função molecular (36%) e processo biológico (35,8%) representaram a maioria dos termos de Gene Ontology atribuídos, enquanto o componente celular (28,2%) apresentou menor número. A análise de expressão diferencial identificou 1.550 e 1.357 genes com maior nível de expressão nas cultivares Amarillo e Belomonte, respectivamente. A análise de enriquecimento dos genes mostrou que 55,63% pertencem à classe componente celular, seguida por função molecular (26,48%) e processo biológico (20,89%). Esses resultados são o primeiro relato de anotação funcional de A. pintoi que irá fornecer uma importante fonte de informação para avanços nos estudos de expressão, silenciamento e edição gênica nos programas de melhoramento de Arachis.
650 $aArachis pintoi
650 $aGenome
650 $aLeaves
650 $aTranscriptome
650 $aFolha
650 $aGenoma
650 $aMétodo de Análise
653 $aAmarillo
653 $aAmendoim forrageiro
653 $aAnotação funcional
653 $aBelomonte
653 $aCacahuetes forrajeros
653 $aForage peanut
653 $aHojas
653 $aRNA-seq
653 $aTranscriptoma
700 1 $aFORMIGHIERI, E. F.
700 1 $aGARCIA, A. L. B.
700 1 $aMARGARIDO, G. R. A.
700 1 $aCAMPOS, T. de
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